FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882061
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAT7301.1160646E-611.9281991
CTCCCAT6203.3216198E-511.7037431
CTCAGAT4450.00471571311.4144361
CTACGAA5350.001497828810.8505731
CTCAAAG9151.486942E-610.3095261
GGAACGA6950.00122946149.3966741
CTCAAAC6600.0084997458.795541
CTACAAT9151.7280824E-48.7234461
CTCATTT9352.1841936E-48.5368481
CATGGAT9452.4505E-48.446511
CTCAATG7850.00371555478.3193481
GTCAAAT8800.00112926188.2458181
CTTTCTT23655.456968E-127.977351
TCCTGAC8550.0082342947.6139642
CTTCCGT10900.0011318827.32289171
CTTCTAT11200.00150968627.1267431
TTTTGGA11600.00226586736.85796457
CTTCCTT17201.1589109E-56.7500661
GATTTCG10800.00864097856.69632865
CTTTCAA16303.9864928E-56.6775951
Produced by FastQC (version 0.11.5)