FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484645
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGA6705.93993E-611.8518431
CCCCCAT11252.263867E-68.9834581
CCTCGGA7750.00347233388.3831811
CTCACTT12206.7567926E-68.2839261
CTCAATT18501.2350938E-98.194371
CCCAAGG12457.776993E-57.5377551
CCCATGG13552.740672E-57.45859051
CATCGAT18205.2701944E-77.13952161
CCTCAAT18305.7725265E-77.1005081
CCCAAAT18356.0400816E-77.08116051
CTATAAC17401.9778854E-67.05289941
CTCCAAT21502.2691893E-87.0509691
CCTAATG11450.00201736976.93513971
CTCTGCT25401.6425474E-96.82096051
CTCCTCT16153.7795086E-56.7048141
CCCAGAA20555.5007877E-76.67436271
ATCCAAT30604.7293724E-116.6054831
CCTCAAC12200.0039283366.5087991
CTCAAAA36650.06.49991941
CCCACAT16856.8073685E-56.42627531
Produced by FastQC (version 0.11.5)