FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941389
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT7850.00372213268.3178221
CTCCTGT7900.00394158258.2651781
AATTGTA9600.00298790337.46977427
CAAAGGA15551.5615301E-46.5318231
CTTCAGC12250.0038850316.51468471
CTCCGCC15701.7690944E-46.46941761
CTTTGAT14806.082743E-46.3726261
TATTGTT14450.00368291565.95514157
AATATTG15700.00142540035.9377575
CTGGAAG18802.8434693E-45.78855561
CTTTTCG17657.98189E-45.75466631
AATAATC15150.006228235.6799875
CTTCCAG16850.0028809785.5973211
CTTGAAA16900.00298396615.58076141
GTTTTCT16000.0099968455.4412421
CTTTTAT18000.00627300335.23971461
ACATTTT17900.0067272965.208534