FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915155
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2650.0021777416.276237
CACGTCT2800.00314990915.4000829
GCACACG4250.003587530711.840166
TTAATAT11955.565362E-79.0234333
TTGCCCC6400.00713232168.9858358
GTTACAG6550.0073645868.9507721
CACCATA7850.00339823578.4020391
CTTCGAT9950.0034287687.3652711
CTTCAAA16505.40339E-67.106371
CTTTTAT15351.1566467E-46.68392321
CCTTTGT12350.00380647626.527361
CTCAAAA13600.00146136336.46627471
CTTTCAG14200.002375946.19305131
CTTTGAA16954.1792323E-46.05299231
CTTTTGA15800.00779109775.56590751
TTTGTCA15700.0089813875.49452358
TATTTCA17450.00485374035.3554557
Produced by FastQC (version 0.11.5)