FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264399
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGAG3250.00826231513.3175645
CTACGAG4500.00487973611.3628381
CTCATAT10751.0376662E-69.5130741
CTCAGAT6600.0080535538.8541591
GAGATAC10156.0488088E-58.5284895
CCCAGAT9600.00249470847.6090431
GAGATTC10457.698823E-47.5933475
CCCATAT11803.0057895E-47.42848971
CCCCAAA9850.00320878957.41592031
CTCAAAG10200.00451086047.16145231
CTACAAT13401.6646444E-47.08663131
CTTTCAA25151.022272E-96.970671
CTCTATC11550.00195002156.95683961
CTCCATT16958.471365E-66.8952751
GTCAGGA10600.0065531096.8912091
ATTTGTG13000.00104942276.6587826
CATAGAT11050.009790566.6105721
CTTATAA19207.644279E-66.46768671
CTCCGAA12850.0059607216.2530351
CCTCTGC12950.0064605886.20474861
Produced by FastQC (version 0.11.5)