FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587340
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGTAG2850.003437505615.1980325
CCCATCG3609.626262E-414.1019311
CCCAGAT4201.9617256E-413.8141371
CTTAATT3750.001310253313.5378531
CCCATGA4553.861495E-412.751511
CCCAAAG4400.004348849411.5379431
ATCAAGT5100.001007746111.3763481
CTCAAGA4850.00897276510.4674131
CGAAAGA5750.002738255310.0903251
CCTCGAT6607.6891313E-49.8896661
CTTATTT9402.3239013E-48.4868761
CTTCTTT19703.796231E-97.73100761
CTTTATT11301.9758905E-47.70168641
ATTTTGC11251.9799452E-47.7003376
CTTCAAT8550.0080338297.6341281
CTTTCAT10650.00733803356.80978541
CTTTTTC24154.8236718E-55.40553141
Produced by FastQC (version 0.11.5)