FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831546
Sequences flagged as poor quality0
Sequence length100-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTAG3000.004451349414.6196631
CCACAAC4704.753277E-412.44226551
CTTACAG5200.001110689511.2458941
CTCCATT8606.0897764E-711.0497461
CTCACAA4650.006193860411.0040461
CCCAGAA5750.00256678410.17021
CCTCAAG5750.00256678410.17021
CCTTGAA8901.0970007E-59.8559521
CTTCAAT12801.203116E-79.1372891
CTTACAT7250.00169025379.0742731
CTCATAA9101.5031979E-48.836061
CTTATAT13653.218247E-78.5683011
CTCAAAA12801.1763732E-68.5662081
CTTATGT7700.0029200358.5439591
CTTCCTT14651.040753E-78.4823981
CTTCTCT11352.0898846E-58.3724941
CTTTAAC9853.5396873E-48.1632631
CAATGAT11903.7807822E-57.98553041
CTTTCAG12054.4207227E-57.8861251
CTCAAAT8350.00606136687.87886051
Produced by FastQC (version 0.11.5)