FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430721
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCT850.002807999733.9481245
CCCCACT1550.001827505323.3413681
CTCTTAT2308.0571644E-418.8760621
CTTCGAA3650.001086235513.8769241
CCCATGC3850.001624313313.1560431
CCTTGAT6808.7340166E-510.6409181
TTGATTT9552.417255E-710.5754623
GAATTTG6050.0043540259.5391434
TGATTTC8200.0057878747.9177784
CTTTTTT12500.0049167326.3675251
Produced by FastQC (version 0.11.5)