FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916179
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAGT5306.7650217E-613.6635431
ACGTATA4850.00941192910.4011374
CAACGAT7900.0040072518.2500121
ATCCAGC11850.0029431976.6895816
CCGACGA14103.450855E-46.6767241
GCTTCGG15900.0095212455.4654171
Produced by FastQC (version 0.11.5)