FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10609149
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAT93300.06.52789931
CCTCAAT82700.06.48786831
CTCAAAT85550.06.18697931
CCCATGG58350.05.9645291
CCCACGA44050.05.92560051
CTCAAAG102800.05.85410831
CTCAAAA146800.05.72938161
CTCAGAG36002.3483153E-95.6393791
CTCGAAT40652.4920155E-105.52938651
CTTCAAA192900.05.52536441
CTCCAAT90200.05.46610741
CTTTAAT147100.05.42195371
CTCAAGA70000.05.38618331
CCCACAT71350.05.3858931
CTCAAAC76350.05.3180791
CCCAAAT81950.05.30857661
CTTTCAT208000.05.2985381
CCCAAAC61100.05.22140361
CCCCAAT57150.05.20167731
CTCACGT32201.3072113E-65.17902231
Produced by FastQC (version 0.11.5)