FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957527
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA4051.8189894E-1224.6017484
AGAGCAC5053.8198777E-1119.7290843
AGCACAC4352.4720066E-919.6329215
CACGTCT4554.3201E-918.7699369
ACACGTC4558.821189E-817.2057748
CACACGT4609.992618E-817.0187537
GCACACG5002.581055E-715.6572556
CACGTAT7101.568994E-410.045361
AAGAGCA12851.9187246E-78.8638672
GTTTTAA8900.00149769258.0137151
GAAGAGC12056.0092745E-57.69449571
AAATTGA11450.0023398216.8372294
GAAAATT11550.00255701046.77980232
TAGGATC3153.2509597E-56.778173430-34
ATTCAAT16150.00213149145.74109361
GATATTC24153.6964973E-45.00984955
Produced by FastQC (version 0.11.5)