FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904341
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCAAG6650.0010560089.5543221
GCAGGGT8601.2656202E-48.9772768
TTCGATA8000.00593423947.8976682
GAATTCC9100.00218291727.7127446
GTTTGAT11200.00201879676.93351841
CAAAGAT13050.0099518435.9506061
CTTTGAT15200.00767714165.57335421
AGATTTT17900.0086486865.0975874