FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141058
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCT6253.5421588E-511.631021
CCCAAAT7602.2230377E-510.52148151
CTTCGAG7802.960487E-510.2516991
CGAGAAT8851.0970487E-59.8567971
CTACAAG9954.2460542E-69.4976921
CCCAAAA12055.1990482E-68.4457611
CTTTCAT21553.6379788E-128.4331641
CTTTGAT17252.5283953E-98.4282761
CTTGGAA11402.3705925E-58.2896531
CTTCTAT13703.3709457E-67.95918371
CAACCAA8300.0060400477.88246871
CTGAAAT20307.876224E-107.8781541
CTAAAAA17954.5542038E-87.6946161
CTTTTAT26501.8189894E-127.6808621
CTTCGTT9650.002752127.5330441
CTTTAAA15753.0478604E-67.3847741
GTCAGAT9850.00336371737.3800891
CACCAAT9900.00353424727.34281541
CTTTCGT10050.0040926447.23322151
CTCAAAG12355.3583365E-47.06337261
Produced by FastQC (version 0.11.5)