FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452961
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAC1000.006080524528.990671
CTTATAA5658.3499253E-714.1105041
CTTCTAA3150.006504861613.8050821
CCCAGTT3250.00799910813.380311
CTCCATT5501.4313083E-411.859821
CTTTAAG4300.00367832211.7985291
CTTCCGT4750.0077210310.6807751
CCCTGCT6154.0337775E-410.6063441
ATCATCG7350.00212657348.8462773
TTCCGCC7700.00325138268.4413779
GGGGGAA2050.0036131997.3268943140-144
GAAGAAA15451.997952E-57.0140384
TTTATAA12850.0066855566.18231347
Produced by FastQC (version 0.11.5)