FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891763
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT7650.00274224188.6049291
CCCAAGT10100.0040503517.24177271
CTCACTT13150.00102294756.67454671
CTCACAT11050.0096775956.61917641
CTCAAAC24452.9378498E-86.58127551
CTCCGAT20653.4050136E-66.3755651
CTTTAAA45350.06.1287591
CCCCAAT13200.00777990566.09515861
CTTCGAA16954.2974355E-46.0412191
CTTCAAT42700.05.9952381
CTTACAT17154.995764E-45.9707681
CCCTTAT16050.00146814315.92426631
TCAAACC20301.3895359E-45.694293
CCTCGAT27551.8820429E-65.5752451
CTCGTAA15750.0077027525.57271671
CTCCCTT17150.0032323475.54428431
CCCAGAC16000.0091608975.48564241
CTTAAAT33658.372808E-85.43401961
CTCGTTT18850.0016621475.432291
CTTTAAT48555.456968E-125.4234981
Produced by FastQC (version 0.11.5)