FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734495
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAA11302.4022484E-68.941536
CTCAAAG9300.00192031977.8135111
CTTAAAA11502.3685012E-47.5825031
CACAAAA11100.00135165597.2011011
AATATTA17702.5858972E-66.9311985
CTTTGAT11700.00235752066.83181331
CTTTCAT13150.00109837096.6310861
CTTTTAT14300.00282453426.09781651
CTTTTCG14500.00329904216.0137091
CTTTATC14700.0038437665.931891
CAAAATA17750.00522036855.32199141
Produced by FastQC (version 0.11.5)