FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708664
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAT8350.00657499267.8086841
GTCCGAT10709.467557E-47.44784641
CCTCGAC10300.00537578857.0337121
TCGAATT11850.0028624586.708122
GTAACCG12100.00359703836.5635638
GTTCAAG16152.603719E-46.28025531
CCCAGCT14800.0042957555.87411
CTTCGAT17557.5730245E-45.7792671
ATCCGAT15150.00556852855.73839471
CTTTTAT38754.2382453E-105.6088181
CTAAAAT20308.180132E-45.3532441
CAATGAC19200.00236003875.2826111
CTTTGAT27601.3495188E-55.24979971
CAAAGCA22706.539913E-45.1064131
CCTTTAT27205.9120997E-55.06065231