FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5099902
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAC23801.5252226E-86.80251651
CTTAAAT44651.0913936E-115.6037831
CTCATGG37003.1777745E-95.56903741
CCCAAAC26906.57119E-65.47143941
CTCAGAC17550.00395046875.4511751
CCCAGAG24404.5451445E-55.4288341
CTCAGTT21752.8656927E-45.41358041
CTCACGA20708.6264417E-45.3326711
CCCCGAT30501.9265717E-65.3081931
CTCAAAA60050.05.26962041
CCCACGG29706.6645007E-65.20339441
GTTAAGA27154.440779E-55.1500051
CCTCACT20200.00366543355.10035661
CCCAGCT43405.1913958E-95.08692551
CTCAGAT27555.652332E-55.07523161
Produced by FastQC (version 0.11.5)