FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871583
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGT1851.8536294E-423.4697911
CTCCTAT4550.005679478411.1331061
CTAAAAC5200.001208478111.1331051
CTCCGTA4600.00616074611.01209451
CCCAAAT6808.7491615E-510.641941
CTTCGAA6154.0997253E-410.5900281
CTCAGGA5750.002790880210.06821
CCTCGAT6506.826301E-410.0197951
CTTTAAT14551.09139364E-109.9471051
CTCAAAA11352.395791E-79.5636811
CCCGTTT7753.271994E-49.3374431
CTCAATT6250.0055574079.2627451
CTTTGAA15654.3837645E-109.2479481
CTTAATT7050.00143607599.238111
CTCTATT7200.001739799.0456491
CTTAAAT9854.0955663E-58.8160641
ATCCGCT11703.710682E-68.6590831
CTCATAT7600.00284234068.5695621
ACAACAT9502.7785185E-48.3488183
CTTTAAA14751.3663339E-78.3403951
Produced by FastQC (version 0.11.5)