FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356837
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACAC7502.3401159E-49.655281
CTCCGCT10407.687049E-58.3555321
CTCCGAA12951.43778725E-57.8286061
CTACATA8550.0081570387.62258961
CTACCAA10950.00121564787.2745261
CTTATGA13251.6132758E-47.10482931
CTATCAT14506.422874E-56.9917551
CTTCCCG18758.182251E-76.9518021
CTTTAAG11600.00223718076.8669021
CTTCAAT23251.31294655E-86.85213471
CTCTTCT13650.00174463556.3661191
CAACCTT12700.00577558446.2721311
CTTCGTT14400.003179596.03455071
CTTGATT16100.00171510135.84714171
CTTTTAA19901.00702135E-45.822281
CTTATTT21555.1568175E-55.7125211
CTTTAAA20301.341324E-45.70755531
CCCGCCG18050.00108820515.61664531
CTGCAAT19554.9590797E-45.55610751
CTGCTGT20056.965654E-45.41755151