FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731212
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT22050.010.2034131
CTCTAAG10104.842532E-710.0600241
CCCAAAT18107.2759576E-129.2223511
CTTAAAA30600.08.77551651
CCCAGGG7600.00277239958.5945141
CCTCAAT27900.08.5842461
CTCACAT13553.2241405E-78.5698471
CTTAAAT21253.6379788E-128.5383411
CTCAAAG32400.08.5119871
CCCTGTT22309.094947E-128.1363111
AGATCCC8150.0053859637.9845556
CTCTCGT14559.505311E-77.9808541
CTTATAT36400.07.9753731
CTCCTTT45650.07.9491671
CTCAATT16601.0335498E-77.86967561
CTTAATA18459.1840775E-97.86730531
CTCCCGT14801.2294531E-67.84604261
CCCGCAT18601.0675649E-87.8038591
CTCAAGG20551.0495569E-97.76968241
CTTAGAT9400.00216394947.72083951
Produced by FastQC (version 0.11.5)