FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290421
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT19303.1468517E-108.2419281
CCTCGGA10306.4411433E-47.7218071
CTCCCAC14957.1754254E-46.28732061
CTCAAAT19155.9121146E-56.0410811
CCCGTAT16804.4436584E-46.0253491
CTCATGG17055.3706835E-45.93700081
AGCTTTA20101.2613766E-45.7325644
CTTCGGT20408.9757575E-45.3164841
GGTACAT17700.0053703825.3104771
CAACAAT30652.951927E-65.18986031
GTCCAAA18150.00720657275.1788121
CTTCGAC18350.0081900485.12236741
CCCGGAT32501.6536687E-65.11691141
CAATTCT26955.250862E-55.09751221
CCAGAAT29951.0660813E-55.0697421
CCCGAAA28752.8349928E-55.02985671
CCCAAAA25951.5813249E-45.01531931
Produced by FastQC (version 0.11.5)