FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554004
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT13301.7479663E-47.059571
CTCAAAT29401.8189894E-116.8785551
CCCAAGT13200.0012323136.56589371
CTCAGCT18806.589855E-66.53096871
CTCAGAT20253.0409483E-66.41998531
CTCAGAA23901.6831473E-76.34611461
CTCTTGG17951.6141127E-46.03550051
CTTACAC14700.0041257435.8959041
CTCCTAT21003.6283647E-55.8467721
CTTCAAA63100.05.83750631
CTCCAAT29704.5980414E-85.836351
CTACAAT33501.7034836E-85.60550981
CTCAGGA21002.2687546E-45.50284431
CTTTAAT49900.05.5000871
CAGAGAC18450.00148721185.4804751
CTTCAAT51400.05.4800941
CTTGGAT25251.8155964E-55.43473961
CTTTTAG21653.5018937E-45.33763171
CCTCGAA20308.534497E-45.336811
CAAAAGG44701.1277734E-105.33203461
Produced by FastQC (version 0.11.5)