FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258032
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT6705.621401E-611.9093881
CTTGGAC6300.00582099439.2113021
GTTTAAA8750.0098734337.4611541
CCCAGAT10750.0080308136.74781371
CTCAAAG11950.00300300246.67723041
CTCAAAA14354.1922778E-46.5714771
ATTTTGG13700.00188151396.32347636
CTTTGAT19851.4258911E-56.2124081
GAATTTC20552.6152149E-55.97217235
CTTCAAG15800.00134128025.96839861
CAAGAAT16650.00265343465.63669254
TTCGTTT15700.0085142245.5216692
CACCGAT17650.0049950575.3428161
TGACGGA20650.00107593785.24425368
CGATTTC22706.865E-45.08849534
Produced by FastQC (version 0.11.5)