FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039396
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGCA5400.001686987610.6972228
CTCAAAT9151.5358437E-610.2840771
CCTAAAA8256.0989405E-48.7738281
CCCAAAA10659.0821297E-47.47628931
CTTATAT12657.3975755E-46.86647371
CTTTGGA11600.00224587796.86400751
CAAGATA10800.0087766656.68576344
CTTATAA13050.00105386166.6560081
GGAAGAT18504.9373066E-66.651511
CTTCTAT12800.0062901616.22050671
CTTTCAT21053.624733E-55.8457451
CATTGAT18000.00105532295.6298731
CTTCTTT32502.8657632E-75.34528641
CTTCAAA18250.0075735355.1561261
CTCTTTT24503.5615385E-45.02256871
Produced by FastQC (version 0.11.5)