FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537859
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAT21303.6379788E-128.4827231
CCCAAAC14457.8146495E-67.50236651
CTCAAAA35600.07.30848551
CTTCAAT37000.07.227281
CTTTAGG11700.00250197066.79487851
CTCAAAC20704.30392E-66.2845911
CTTCGGA26953.8746293E-86.1679941
CTTTAAT34751.70985E-106.03139931
CTTTGAT44850.05.9623041
CTTAAAC15850.00145767335.92773771
CTCAAAT24501.7324655E-65.8998211
AACGGAC17608.1709406E-45.7452342
CTCAGCA15650.0081906065.5416841
CCCCGAA17000.003355385.5267441
CCCAAAG19705.6521746E-45.50300461
CATTCAC20157.655529E-45.38010931
CTTAAAA33751.1546217E-75.3535411
CTCCGCA23001.4237051E-45.34190231
CAAAACT23001.4237051E-45.34190231
CTTTAAC18950.0020648625.3394151
Produced by FastQC (version 0.11.5)