FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433243
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT12100.020.7823939
CACACGT12900.020.035347
ACACGTC14400.017.9483268
GAGCACA20100.014.5963514
AGAGCAC18150.014.2402023
GCACACG19950.013.3055176
AGCACAC20550.013.2569565
GAAGAGC36050.07.9611371
AAGAGCA42450.07.07652042
CTCTCGT11150.00211019336.90580561
CTCAAGT13804.026748E-46.59417961
CCCAGAT29703.3833203E-106.36361361
CTCAAAA46650.06.1521761
CCTCAAT26653.763671E-75.7785911
CTCCGAA26904.509493E-75.7248871
CAACTGT26301.7973325E-65.5893341
CTTCGAG17650.00126112355.55238771
GTAAGAT21901.1022375E-45.433771
CCCCAAT16900.00456484645.38459631
CTTAAAT27353.6886668E-65.37475251
Produced by FastQC (version 0.11.5)