FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295134
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT7108.010029E-712.2613871
CTTAAAA16300.011.126771
CTCCACT7752.821177E-510.2969281
CCCGATT5750.002735095310.0934321
CTCAGTT6607.6807674E-49.892711
CTCTTCT15453.092282E-119.8606951
CATGACT5900.0035079659.7946353
CTCATGA7000.00131628069.3274131
CTCCAGT7400.00218322438.8232291
CTTGTAT14307.9837264E-88.62441351
CCCAAAT9302.068145E-48.5807731
CCTCGAT11151.8413159E-58.4583421
CTCAAAG11302.1780093E-58.3460631
CTTTAAT18309.0221874E-108.3250131
CTTCAAT22107.2759576E-128.2066221
CTTGAAT13101.6358908E-57.75306561
CCCAGAT10709.325972E-47.45805551
CTAAATT9800.0032653827.4027081
CTTTCGT10800.00102967287.3891
CCTCTAT10000.0039772297.2546541
Produced by FastQC (version 0.11.5)