FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378535
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG7652.5968126E-49.5558381
ATTCGCT9954.7364032E-58.7085398
CTCAGAA7800.00328280968.4348641
CTCCGAT11402.001844E-47.69496351
CTTGTCT10950.00109954327.3435951
CTTCGAG10050.00387641557.2738461
ATTAAAC11250.00146479717.14776561
GTTCGAT10500.00593511156.962111
CACGAAG10600.00650612036.89642951
CTCATTT15059.2457E-56.80021
CTCTCCG11950.00276921066.72906881
CTTGATT18504.237736E-66.7174951
CTTTGGT14403.9814698E-46.59949971
TCCGATT15601.7358985E-46.47952652
GTTCAAT14855.78667E-46.39951561
TCGATAT13700.0018774076.32481533
CTCCATT15207.671062E-46.2521581
CTTCAAA26902.9433068E-86.250371
TTTGGTT12950.0072920536.1328562
CTTCAAT22752.539089E-66.10523461
Produced by FastQC (version 0.11.5)