FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022714
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTATTCGCCATCTCGTATGCCGT20700.1023377501713045TruSeq Adapter, Index 9 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT8900.033.9912157
CACGTCT9550.030.9234479
ACACGTC10450.027.5709138
GCACACG14450.021.4342386
AGCACAC15400.019.6442765
AGAGCAC16050.017.9538173
GAGCACA18900.015.6276584
GAAGAGC28650.010.1129031
AAGAGCA29500.09.2812962
GTCACTA6850.09.06214320-24
CTATTCG8700.07.793541425-29
CACTATT9350.07.097452625-29
AGTCACT9050.07.018707820-24
GATCTTT19751.922508E-66.60154961
CTCGTAT9700.06.545334335-39
GATCCTT17751.898545E-56.5292321
GTATAAG13550.00160218616.41478971
TTAAATC14856.900138E-46.30759672
TCACTAT11000.06.29513125-29
ATTAAAT19901.0043651E-45.8238121
Produced by FastQC (version 0.11.5)