FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870060
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT10800.013.3775727
CACGTCT10857.2759576E-1111.9843319
ACACGTC12155.329639E-1010.7020588
GCACACG17956.006303E-98.0489016
AGAGCAC18708.1113103E-76.95650633
AGCACAC19802.851375E-76.93201455
CCCGCAC19356.4956046E-56.00220631
CCCCAAC14600.00361443685.9662341
CCCGCGA14600.00361443685.9662341
CACTTGT18150.00113369985.59916541
CTCTTAT20751.7782381E-45.5972381
CTTAAAT21152.336578E-45.491381
GAGCACA23351.797098E-45.25963744
CTTCAAG42604.5747583E-95.11191461
Produced by FastQC (version 0.11.5)