FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503301
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTGACTTCGATCTCGTATGCCGT6510.12934605732951057TruSeq Adapter, Index 4 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2551.5097612E-1029.8616438
GCACACG2851.8189894E-1129.1472516
AGCACAC2702.9467628E-1028.2026625
CACGTCT2753.6379788E-1027.6898889
AGAGCAC3003.274181E-1127.6898863
CACACGT2855.511538E-1026.7183137
GAGCACA3151.7607817E-924.1737124
GAAGAGC5908.703864E-915.2908811
AAGAGCA6303.2962635E-713.1882812
TTGTGGC4353.9126747E-412.7309849
GCCAAAC4500.007136008710.79511
CGAAAAG8050.00694736757.75867841
ACTCCAG2458.538694E-46.781466515-19
CAGTCAC3400.0055592946.1232711
CTGACTT4302.452336E-66.11850625-29
AGTCACT2900.0056591575.730308520-24
CACTGAC4607.700164E-65.719472425-29
GATCTCG3409.007144E-45.70142535-39
GGAAGAA17109.359567E-45.6816311
TCGATCT4351.17287425E-45.411205330-34
Produced by FastQC (version 0.11.5)