FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189839
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTT9501.2914825E-912.2272321
CTATGAT11953.6379788E-1010.9354431
CCCATAC7251.6205604E-410.0136811
CTCACGG8104.5486428E-59.8591491
CTTAGAA6350.00616929039.1463551
CTCAAAT9552.7426839E-59.1224121
CACATGA8004.356348E-49.0748991
CTCAAAC8356.678019E-48.6945141
CCCTTGT9201.8248531E-48.6803381
CTTCAAG17754.8930815E-108.5891991
CTCTTAT10306.6629684E-58.45815851
GTCATGA7850.00370090628.3234741
CTTTGAT18701.3587851E-98.152851
CTCTGAT8600.00839848357.597591
CTATAAT14851.0758209E-57.33325151
CAACAAG15451.877006E-57.04846531
CTCCTGA11350.0017300487.03604461
CTTCGAT11700.00238344246.8255651
CTTCAAT18153.499803E-66.7999241
CTTCTCA10750.0079659996.75341271
Produced by FastQC (version 0.11.5)