FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1652225
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT5900.00342465779.8234271
CTCAATT9753.4973538E-48.1735951
CTTAAAT11350.00176969467.02137041
CCCAAAC10950.0097139936.61623531
CTCATAT12050.003460286.5878723
CTCTGAT12400.00448229686.4268191
ATTTGCG15108.278039E-46.2130656
CTTCAAT25709.45729E-86.2017551
CTTCAGT13000.00732696576.13019661
CCCGCCG18051.6673356E-46.0205911
CCCGCAT15750.00131170455.97981641
CTTTGAA29854.775211E-85.8249471
CTTCAGG21856.3220825E-55.63666871
CGCCGTT18400.00138054045.51233051
CCCGGAT22358.904475E-55.5105691
TTTACCT21102.452077E-45.47238977
GATATAG17450.00464342955.37634855
AGATTTA17500.0048024235.36098774
CTTTGAC18950.00200150145.3523421
CACCGAT21753.5732164E-45.32949161