FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200696
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAG5300.001423004810.9180276
CCTTCCT10951.441007E-69.2862351
CTCAAAT11502.6740352E-58.2105311
CTCCAAG8300.00608718447.87571861
CTTATCG10255.81967E-47.79461341
CTCATTT13251.8736635E-57.6742851
CTTCAAA22004.083631E-97.26316261
CTTCGAT13150.00110592346.62798071
GTTTGAT14303.9553922E-46.60287481
CTTAAAT12350.00418255666.46921351
CATTGAT19551.1046923E-56.31579351
ATTACAA16503.3200713E-46.1626831
CTCAAAA16603.588883E-46.1255591
CTTTTAT28651.8955689E-86.08432441
GTTCAAA14500.00332129656.0108931
CTTTTAC19758.641806E-55.88408141
CCTTGAT16250.00184810485.810531
TCAAATC15000.005072235.78655433
TTCAAAT25051.5419297E-55.48694042
CTTTAAA22801.1562425E-45.4155161