FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598225
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT9201.9221874E-48.6392661
ATGACTA8000.0048123758.0847694
CAATAGG8050.00484726168.0782761
GTTTAAA13001.5592635E-57.7813811
CTCAAAT12951.2504066E-47.2534661
CCACAAT14354.3992262E-46.5458111
TCAGATT19058.668079E-66.41592552
CCTGTGT12750.0061569416.2338241
GGTTGAT15259.188362E-46.15950161
CCTCGAT14150.00267970456.1276911
CTTACAG13050.0078362966.09051751
TTTGCGA14250.0030659246.0544092
CAAAGGT19851.00292724E-45.8241361
CTTCAAT26351.0328185E-65.75851771
CCTGATC15650.008206775.5403721
CTAAAAT17000.0033623055.5254351
AACATTT27452.421979E-65.49869973
CCCGCAG17400.00442448965.39841371
CCTTTAC19350.0026891565.22780131
ATCAAGT18100.00702874465.1896351
Produced by FastQC (version 0.11.5)