FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695672
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTGT4050.002748459612.2683591
CAAACAC6153.8274884E-511.5416621
CTTCGAA4350.004689947711.4222651
CCCCGGT4800.00974779510.3514281
CCCTTGT4800.00974779510.3514281
TCCGATT5850.0039243569.6614712
TCCTGTG6400.0082191838.8311882
CAAAAAA18401.0673466E-77.3295821
CTTCAAA11703.7833132E-47.28012471
AAGATAA12609.3289395E-46.72707375
CTTTCTT20401.911046E-45.56715441
CAAAGAA18100.00144796265.490261