FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091719
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACAGT9803.8315608E-58.867791
CTTAAAC9703.323596E-48.212611
CCCGAAT15953.902429E-67.26473051
CCCACGA10700.00780475846.7682511
CATAGGA12150.00382930586.5245967
CCCAAAT16655.518273E-56.52434971
CTTCGAA15158.2618947E-46.2142821
CCTTGAG12900.00679194236.1753731
CAAGGTC17651.3191495E-46.1246868
CTTGGGA14350.00305788456.05605171
GTTTATT28708.1514554E-75.55138061
GTTGAAT21603.2581188E-45.3644661
CCAATCT17650.00539394475.3084413
CACATTT17800.0056297525.28912161
CTTTAAT30402.449986E-65.24094151
GGTGTAT18300.007785365.14461041
TGATACA25451.2260312E-45.09745174
Produced by FastQC (version 0.11.5)