FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753571
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTCT2650.002060437316.4111391
CTCAGTT2750.002638485715.8143681
CTCAAAT6602.15623E-814.2768611
GACTTGG4100.002642344512.3331886
CCCCTTT5601.6942881E-411.6489761
AACCACT4350.004112958411.6251562
CACCTAT4550.00561018511.1511571
CTTTAAG7051.24022E-410.2812091
CTTTGTT10458.198047E-69.0169651
CCCATAT6500.00756671848.9209261
CTTCAAG8356.7785056E-48.6805421
CTCATTT9250.00186705327.8359481
CTCCTTG9300.00196874687.79381941
CTCCTTT14054.1787134E-57.2224581
CTTAAAA10350.0055992787.0031431
CATGAAG10950.0096549386.61940861
CTCTTTC14504.7941483E-46.49843261
CTTTGAT12500.00484122246.37846231
CCATCAT13700.00179586296.34883451
CTTCTTT24151.2643704E-66.00269371
Produced by FastQC (version 0.11.5)