FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413214
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGCT2550.001502463117.2018011
CTCAAGG3250.007551903413.4967981
AAGCTGG4250.003437035711.9056545
ACAAAAC4907.4236013E-411.7972372
CTTAAAA5200.001107421411.2473321
CACAAAA6000.00364107279.7476871
CTTCAGG6500.00704048578.9978661
TTCAATT7450.00239912588.7291642
TCATTAT8508.294635E-48.5040393
AGGGCAA4800.00189178476.02369458