FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016959
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC4350.024.910068
CACGTCT4101.8189894E-1224.6670349
CACACGT4151.8189894E-1224.369847
TAACCCC2857.3228493E-620.277669
GAGCACA6201.8189894E-1218.6423684
GCACACG5953.1468517E-1016.9974546
AGAGCAC6751.09139364E-1016.053153
AGTGAGT2850.003339064515.2660411
AGCACAC6902.564775E-914.6572245
GTGAGTA3350.00988383412.9606632
ATAACCC6353.2312855E-612.5138728
GAAGAGC10204.0199666E-911.3746981
TATAACC8205.4935055E-59.690627
CTTCGGT8900.00127177628.1476071
AAGAGCA14205.9807335E-67.64405252
GTTCTCT10050.00419193467.2152931
TCACTCG3451.7052207E-67.120202525-29
AGTCACT4251.3449062E-76.800184220-24
GGATACC11900.0028828966.7029471
CATAAAC11900.0028828966.7029471
Produced by FastQC (version 0.11.5)