FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324956
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAG6400.0062457249.1327921
TGACTAC6400.00692098359.019373
GTTCGAA7500.00231257458.7674811
CAAAGAC13352.3152643E-78.7565361
CTTCTAA10708.6480926E-47.51108171
ATCCAAC10608.9395203E-47.48777876
CTCAAAG11953.4678035E-47.3368041
GTTCAAT11500.00185870836.9885721
CTTTAAT17008.823239E-66.8764561
AATTGGT10800.0088440086.6807637
CTTCCGT15851.8289544E-46.45345641
TAAATCT14605.516559E-46.42475653
CAACGAT14906.06659E-46.37456661
CCCTGAT13850.00185893236.33031131
CTATGAT12750.00548389236.30341771
CACCAAA14100.0022730646.2180721
ATCAAAT20051.4899482E-56.19481231
CATCAAT26101.0833173E-76.1585111
CAACAGG13500.0099148665.9532281
TCAAATT17206.170544E-45.8724132
Produced by FastQC (version 0.11.5)