FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396703
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA3250.00818016613.3378511
CTCGCAT6154.1656377E-410.5726861
TCGAATT9000.00150479618.0104512
CTCCAAT12004.1392935E-47.22466951
CTAAAAT12607.231148E-46.88063761
CTCAAAA19404.6122164E-45.5860841
CTTTAAT16900.0031392865.5574381
CTTTTCT31351.4132092E-75.5308471
CTTTGAT20358.7688485E-45.3253091
TTTTTAC24253.2505917E-45.05401666
Produced by FastQC (version 0.11.5)