FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685925
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2653.6379788E-1232.626957
ACACGTC2701.8553692E-1029.3520438
CACGTCT2955.18412E-1026.8645829
AGAGCAC4807.186827E-918.0127943
GAGCACA4851.5852311E-716.3415034
AGCACAC5756.654409E-815.0367685
GCACACG5851.3234403E-613.5480846
GAAGAGC9251.775632E-610.1668771
AAGAGCA9803.891797E-69.5612952
AGTCACG2601.7329276E-68.34047320-24
ACGTCTG3652.0939455E-45.921582710-14
CAGTCAC3559.0135203E-45.701280620-24
CTCGTAT3150.0089020625.50003935-39
CGTCTGA4303.2861988E-45.36155710-14
CGTTATA3600.00671984775.21007830-34
Produced by FastQC (version 0.11.5)