FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879041
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2508.200368E-825.975277
ACACGTC3106.5363383E-720.9477988
CACGTCT3403.3812063E-516.97739
GCACACG6202.5041154E-612.8014336
AGCACAC7702.2715558E-611.2447065
AGAGCAC7101.3921795E-410.1647823
CCCCCCC1950.0021956849.157342145
GAGCACA7850.00390228378.2742614
CTTTTGA11100.00138892687.18303731
CTCAAAA10650.0073867636.805951
GAAGAGC12300.00409243546.48225261
CAAAAAA22302.0813477E-66.17571351
Produced by FastQC (version 0.11.5)