FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494172
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1501.5277219E-632.5610167
CACGTCT2152.6228008E-829.2075569
ACACGTC2751.6638325E-417.7605538
GCACACG3202.6736534E-517.4451686
AGAGCAC3306.677856E-414.79896453
AGCACAC3408.359741E-414.3666085
GAGCACA3700.001583027513.2017494
CGGACAT5800.00396516039.6483561
Produced by FastQC (version 0.11.5)