FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460368
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAGG5600.002192411310.3661141
ATTTGCG10558.3834643E-47.53314926
CTTCAAA24106.002665E-117.5272611
CTCAAAT12908.9943176E-46.75002771
CGCATGG13050.00749853676.11640361
CCCAGAT13050.00749853676.11640361
GATTCCC13100.0081584786.0667737
AACATTG15550.00764418635.5761163
CTTTGCT18750.00171609775.41802171
CAAACAA19350.0025495125.2500211
CCTGTAT18050.00647435575.22612861
CTAAAAA18450.0083636725.11282541
CAAAAAC25901.449011E-45.04297351