FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803100
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATTC8800.00124868468.162251
CTTCAAT13402.9118655E-68.0404251
CATTAAT8650.0097223377.4734131
CTCAAAA11653.1444835E-47.3985721
TCCATCT11400.00211520476.9027043
AAACTTT13350.00155578176.43030174
TTCCATC14656.347159E-46.34997942
CACAAAA12600.00578237056.2706811
TCATTAT14958.1403827E-46.22061823
CTTTTAA14350.00334462036.0065061
CTTCAAA16300.00218610195.7285971
CTTTCTT24058.7452245E-65.6745461
GATATAT15150.00639337255.6663065
CTTTGAT15350.0070663465.61520241
CAGATAT15650.0091455725.4852733