FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824509
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA3100.00591122614.0078871
GTTAGAA3200.007294234413.5701421
CTCAACT3350.009872382512.9625231
GGTTCAT6950.00125894189.3721841
CTCAAAG8659.771959E-48.3669471
CCCAGAA7950.00426345048.1932931
CTCTTCT9100.00161336067.9531961
CATTTAC8300.00647108777.82161245
ATCGCTT8400.0069915487.7543671
CGAAAGA8600.0086290997.5740321
CCAAAGC10200.00507920387.07397372
TCTTGTT10250.00534832577.03647857
CTTCTGT13050.0010547466.6550891
CTTTTAT12050.0033529886.6067631
TGGAAGC15057.9949177E-46.2299727
CTTTCTT21050.00134197585.15729861