FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963707
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCATT11602.6706402E-810.0758531
CCCACTT11001.3278623E-79.9613541
CTCACAG8901.1081507E-59.8494291
CCCGCAT16751.4551915E-119.5946181
CCTACGC7550.002746288.6032597
CTCCAAT15904.467256E-88.2698041
CTCATGT8850.00111943818.254231
CTTAAGA9350.00192550557.8128271
CCCAAAT14356.0852162E-67.6358811
CTCCGCT16305.831789E-77.6187051
CCCAGAT17452.141096E-77.53523641
CTCATGA9700.00276190677.5309211
CCCCGTT10950.00110853947.33834931
CTTAAAT18656.4880805E-77.0503961
CTTGAAC17802.3448247E-66.97667931
CCCATAC14706.861589E-56.95713661
CAACGAG14757.174336E-56.9335531
CTTTAGA10600.0065542766.8915031
CTCAGAA11750.00233751956.8387171
CTCCTAT11850.00255557386.78100631
Produced by FastQC (version 0.11.5)